IgM Affinity Resin – Precision Purification for Complex Antibodies

High-capacity, low-harshness purification of monoclonal IgM antibodies

ChromaGenix™’s IgM Affinity Resin is engineered specifically for the purification of immunoglobulin M (IgM)—one of the most structurally complex and purification-resistant antibodies. With a molecular weight approaching 990 kDa, IgM presents unique downstream challenges. Our resin overcomes these with high selectivity, gentle elution, and exceptional reusability.

Key Features & Benefits

• High Affinity Capture of IgM Subtypes
  Efficiently binds both heavy and light chain subtypes from human, mouse, and rat sources.
• Mild Elution Conditions
  Maintains structural integrity and bioactivity of sensitive IgM molecules.
• Non-Immunogenic, Non-Leaching
  Synthetic ligands ensure safe and consistent purification.
• Exceptional Reusability
  Stable through multiple sanitization cycles—validated for use up to 100 cycles.
• Flexible Formats
  Available as pre-packed columns (1 mL & 5 mL), microspin kits, and loose resin for custom formats.

Typical Binding Capacities

Use Cases

• Purification from:
  • Cell culture supernatants
  • Hybridoma cultures
  • Recombinant IgM production
  • Ascites fluid (non-serum formats)

Note: Not recommended for use with serum samples due to cross-reactivity with other immunoglobulins. Please contact ChromaGenix for optimized protocols in such applications.